FIG 1.

The gene arrangement and RT-PCR amplification of 2-MIB-related genes in NIES-512. (A) Arrangement of 2-MIB synthesis-related genes in the NIES-512 genome. The numbers indicate the estimated sizes of each gene and gaps between the genes. Primer pairs shown with each gene (Table 1) were used for RT-PCR amplification. (B) Agarose gel electrophoresis results of RT-PCR amplified mtf, mtc, cnbA, and cnbB genes from NIES-512. Numbers indicate the band positions of the DNA size marker. The experiment was repeated two times independently with similar results.