FIG 2.

Association between 2-MIB-related genes during the gene expression in NIES-512. (A) Possible transcripts from 2-MIB synthesis-related gene cluster in the NIES-512 genome (a, mtf-mtc; b, cnbA-mtf-mtc-cnbB; c, cnbA-mtf; d, cnbA-mtf-mtc; e, mtc-cnbB; f, mtf-mtc-cnbB). Primer pairs indicated with each gene fragment (Table 1) were used for genomic and RT-PCR amplification. (B) Agarose gel electrophoresis results of PCR amplified gene fragments (a–f) from NIES-512 genomic DNA. (C) Agarose gel electrophoresis results of PCR amplified gene fragments (a–f) from NIES-512 cDNA. Numbers indicate the band positions of the DNA size marker. The experiment was repeated two times independently with similar results.