Comparison of the restriction maps of Tn1721 and plasmid pGFT1. Restriction endonuclease cleavage site abbreviations: C, ClaI; E, EcoRI; EV, EcoRV; H, HindIII; K, KpnI; P, PstI; S, SmaI; X, XhoI. A distance scale in kilobase pairs is indicated below the maps of Tn1721 and pGFT1. The shaded rectangles represent the fip reading frames in pGFT1 and pKM101. The repeated sequence at the integration site of the Tn1721-like transposon in pGFT1 and the corresponding sequence in the fip gene of pKM101 are marked by open rectangles. The numbering of the fip gene area refers to the numbering of the deposited nucleotide sequences of pGFT1 (no. Y11027 and Y11028) and pKM101 (no. U42978).