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Titration-ELISA on native toxins for detecting binding activity of scFv antibodies selected for (A) native TcdA (nTcdA) from C. difficile RT084; (B) CROP domain fragment of TcdA (rTcdA) from C. difficile RT084; (C) native TcdB (nTcdB) from C. difficile RT017; and (D) CROP domain fragment of TcdB (rTcdB) from C. difficile RT017. BSA was used as negative control. Data shown are means ± SD of three independent experiments.
