Fig 6.

Relative expression of IL-8 and TNF-α genes in Caco-2 cells upon co-treatment with (A) anti-TcdA scFv antibodies (1 µg/mL) and native TcdA (10 µg/mL) of C. difficile RT084; (B) anti-TcdB scFv antibodies (1 µg/mL) and native TcdB (10 µg/mL) of C. difficile RT017; and (C) a mixture of anti-TcdA/anti-TcdB scFv antibodies (at final concentration 1 µg/mL) and combination of TcdA/B (10 µg/mL), which were measured by using RT-qPCR. Gene expression data were normalized to β-actin as the reference gene. Data were presented as mean ± SD from three independent experiments. A P value of <0.05 was considered significant (*P < 0.05; **P < 0.01) by unpaired Student’s t test and one-way ANOVA statistical analysis.