TABLE 2.
Amino acid residues of anti-TcdA and anti-TcdB scFv antibodies involved in the hydrogen bonds with the antigens a
| Position | ||||||
|---|---|---|---|---|---|---|
| Heavy chain | Light chain | |||||
| CDRH1 | CDRH2 | CDRH3 | CDRL1 | CDRL2 | CDRL3 | |
| rA-C2 | Ser: 33 | Gln: 54, Ser: 55 |
Asp: 101, Arg: 104 |
Ser: 162, Ser: 164, Tyr: 166 |
Tyr: 183, His: 184, Ile: 187, Leu: 188, Gln: 189 |
Ser: 225, Asn: 230 |
| A-C9 | - | Asn: 54, Arg: 58, Arg: 61 |
Gly: 102, Ser: 103 |
Ser: 160, Gln: 161, Ser: 162, Ser: 164 |
Arg: 184 | Thr: 203 |
| rB-B4 | Ser: 33 | Gly: 55, Arg: 56, Arg: 59 |
- | Ser: 164, Ser: 165, Tyr: 166 |
Ser: 186, Ser: 187 |
Tyr: 226 |
| B-F5 | Thr: 30, Tyr: 34, Ala: 35 |
Asn: 54, Ars: 79 |
Lys: 100, Arg: 102 |
Gln: 161, Ser: 162, Tyr: 166 |
Tyr: 183, Arg: 184 |
Arg: 226 |
| B-F11 | Tyr: 34 | Arg: 56, Arg: 58 |
Tyr: 102, Gln: 103 |
- | Ala: 185, Ser: 186, Ser: 187 |
Tyr: 226, Thr: 228 |
a
CDR: complementarity determining regions; H: heavy chain; L: light chain.