Table 1.
Metastasis-related miRNAs in ovarian cancer.
| miRNA | Expression status (up/down) |
Targets | Model (in vitro, in vivo, humans) | Cell lines/patient number | Target validation method | References |
|---|---|---|---|---|---|---|
| miR-650 | Up | KLF12 | In vitro | CAOV3 cells | In silico analysis and dual-luciferase assay | (62) |
| miR-600 | Up | KLF9 | In vitro, human | HO8910 and A2780/34 | Luciferase reporter assay | (63) |
| miR-140-3p | Down | SNAI2 | In vitro, in vivo | SNU119, SKOV3, CAOV-3, HO8910, and HOSEpiC | Luciferase reporter assay/Western blotting/qRT-PCR | (64) |
| miR-139-3p | Down | ELAVL1 | In vitro, in vivo, human | SK-OV-3, A2780, OVCAR-3/21 | Luciferase reporter assay/Western blotting/immunofluorescence staining assay | (65) |
| miR-338-3p | Down | MACC1 | In vitro, in vivo | SKOV3, OVCAR3 A2780, OVCAR8 | Dual-luciferase reporter assay/Western blot/immunohistochemistry assay | (60) |
| miR-488 | Down | CCNG1 | In vitro, human | A2780, OVCAR3, SKOV3/58 | Luciferase reporter assay/Western blotting/qRT-PCR | (66) |
| miR-328-3p | Up | DDB2 | In vitro, in vivo, human | OVCAR4, SKOV3, OV2008 | Dual-luciferase reporter assay/qRT-PCR | (67) |
| miR-340 | Down | FHL2 | In vitro, in vivo | A2780, SKOV3, HEK293T, A2780 | Luciferase reporter assay/Western blotting/qRT-PCR | (68) |
| miR-331-3p | Down | RCC2 | In vitro | CAOV3, SKOV3, OVCAR3, ES-2, COC1, A2780, SKOV3 | Luciferase reporter assay/Western blotting/qRT-PCR | (69) |
| miR- 30a-5p | Down | SKP2, BCL9, NOTHC1 | In vitro | OVCAR-3, HO-8910PM, HO8910, Caov-3, SKOV-3, A2780, COC1, OV-90 | Luciferase reporter assay/Western blotting/qRT-PCR | (70) |
| miR- 1-3p | Down | DYNLT3 | In vitro, human | OC3, HO8910, ES-2, SKOV-3/60 | Dual-luciferase reporter assay/Western blotting/qRT-PCR | (71) |
| miR- 375 | Down | PAX2 | In vitro | PA-1, OVACAR-3, Caov-3, SW-626 | Dual-luciferase reporter assay/Western blotting/qRT-PCR | (72) |
| miR-598 | Down | URI | In vitro, in vivo | HEK293T, SKOV3 | Luciferase reporter assay/Western blotting/qRT-PCR | (73) |
| miR-32-5p | Up | SMG1 | In vitro, human | OVCAR3, SKOV3, ES-2/38 | Luciferase reporter assay/Western blotting/qRT-PCR | (74) |
| miR-15a-3p | Down | Twist1 | In vitro, in vivo, human | OVCR3, SKOV3 A2780/45 | Luciferase reporter assay/Western blotting/qRT-PCR | (75) |
| miR-208a-5p | Down | DAAM1 | In vitro, human | HeLa, OVCAR-3, HEK-293 T/61 | Luciferase reporter assay/Western blotting/qRT-PCR | (76) |
| miR-125b | Down | S100A4 | In vitro, in vivo, human | SKOV3, A2780, SKOV3ip1, OVCAR 5, CAOV3/70 | Western blotting/qRT-PCR | (77) |
| miR-503-5p | Down | CD97 | In vitro | SKOV3, CaOV3, OV90, OVCAR3 | Western blotting/qRT-PCR | (78) |
| miR-377 | Down | CUL4A | In vitro, human | SKOV3, CAOV3, OVCAR3, A2780, 3AO TC-1, HO-8901/44 | Luciferase reporter assay/Western blotting/qRT-PCR | (79) |
| miR-26a | Down | TCF12 | In vitro, human | SK-OV-3, A2780 27 |
Luciferase reporter assay/Western blotting/qRT-PCR | (80) |
| miR-222-3p | Down | PDCD10 | In vitro, in vivo, human | A2780, HO 8910, SKOV3, MR182/16 | Luciferase reporter assay/Western blotting/qRT-PCR/immunohistochemistry staining | (50) |
| miR-302 | Lower in chemoresistance than chemosensitivity | ATAD2 | In vitro, in vivo | A2780, A2780cisR | Luciferase reporter assay/Western blotting/qRT-PCR | (81) |
| miR-32 | Down | BTLA | In vitro, human | SKOV3/100 | Luciferase reporter assay/Western blotting/qRT-PCR | (82) |
| miR-330-5p | Down | S100A7 | In vitro, human | Caov3, SKOV3/40 | Luciferase reporter assay/Western blotting/qRT-PCR/immunohistochemistry staining | (83) |
| miR-6089 | Down | MYH9 | In vitro, in vivo, human | SKOV3, OVCAR3 16 |
Luciferase reporter assay/Western blotting/qRT-PCR | (51) |
| miR-23a-3p | Up | DLG2 | In vitro, in vivo, human | SKOV3/50 | Luciferase reporter assay/Western blotting/qRT-PCR/immunohistochemistry staining | (84) |
| miR-145-5p | Down | SMAD4 | In vitro, human | SKOV-3/18 | Luciferase reporter assay/Western blotting/qRT-PCR | (85) |
| miR-802 | Down | YWHAZ | In vitro, human | OVCAR3, A2780 CAOV3/35, SKOV3 | Luciferase reporter assay/Western blotting/qRT-PCR | (86) |
| miR-27a-3p | Up | FBLN5 | In vitro, in vivo, human | 293T, SKOV3, HEY, A2780 216 |
Luciferase reporter assay/Western blotting/qRT-PCR/immunohistochemistry staining | (87) |
| miR-19b | Up | PTEN | In vitro, human | SKOV-3, CAOV3, HO-8910 OVCAR3/50, ES-2 | Luciferase reporter assay/Western blotting/qRT-PCR | (88) |
| miR-203 | Down | BIRC5 | In vitro, in vivo | SKOV3, OVCAR3 | Western blotting/immunofluorescent staining | (89) |
| miR-202-5p | Down | HOXB2 | In vitro, human | PEO1, OVCAR3, A2780, 3AO, CAOV3, SKOV3/55 | Luciferase reporter assay/Western blotting/qRT-PCR | (90) |
| miR-205 | Up | SMAD4, PTEN | In vitro, human | OVCAR-3/10 | Western blotting/qRT-PCR | (91) |
| miR-145-5p | Down | VEGF, c-MYC | In vitro, in vivo, human | A2780, SKOV-3/9 | Western blotting | (92) |
| miR-936 | Down | FGF2 | In vitro, in vivo, human | OVCAR3, SKOV3, CAOV-3, ES-2/51 | Luciferase reporter assay/Western blotting/qRT-PCR | (93) |
| miR-141 miR-200a | Up | DLC-1, ZEB2 | In vitro, human | Caov3, SKOV3/11 metastatic SOC | qRT-PCR | (94) |
| miR-616 | Up | TIMP2 | In vitro, in vivo, human | CAOV3, SKOV-3, A2780, HO-8910, ES-2/60 | Luciferase reporter assay/Western blotting/qRT-PCR/immunohistochemical staining | (95) |
| miR-590-3p | Up | CCNG2, FOXO3 | In vitro | SKOV3.ip1, ES-2 | Luciferase reporter assay/Western blotting/qRT-PCR | (96) |
| miR-574-3p | Down | MMP3 | In vitro, human | A2780, OVCA433, SKOV3, CAOV3, SW626/64 | Luciferase reporter assay/Western blotting/qRT-PCR | (97) |
| miR-574-3p | Down | EGFR | In vitro, human | SKOV3, CAOV3/73 | Luciferase reporter assay/Western blotting/qRT-PCR | (98) |
| miR-424-5p | Down | CCNE1 | In vitro, human | SKOV3, HO8910, A2780/83 | Luciferase reporter assay/Western blotting/qRT-PCR | (99) |
| miR-655-3p | Down | RAB1A | In vitro, human | SKOV3/50 | Luciferase reporter assay/Western blotting/qRT-PCR | (100) |
| miR-489 | Down | XIAP | In vitro, human | SKOV3, OVCAR3, HO8910/51 | Luciferase reporter assay/Western blotting/qRT-PCR | (56) |
| miR-217 | Down | IL-6 | In vitro, human | SKOV-3, CAOV3, OVSCAR-3, H08910/15 | Luciferase reporter assay/Western blotting/qRT-PCR/ELISA | (101) |
| miR-34c | Down | SOX9 | In vitro, human | A2780, SKOV3, OVCAR‐3, 3AO, Caov‐3/54 | Luciferase reporter assay/Western blotting/RT-PCR | (102) |
| miR-520a-3p | Down | SUV39H1 | In vitro, in vivo, human | OVCAR3, SKOV3/28 | Luciferase reporter assay/Western blotting/qRT-PCR/ribonucleoprotein immunoprecipitation assay | (103) |
| miR-508-3p | Down | CCNA2, MMP7 | In vitro, human | SKOV3, HeyA8 A2780/130 | Luciferase reporter assay/Western blotting/qRT-PCR | (104) |
| miR-301b-3p | Up | CPEB3 | In vitro, human | HO8910, SKOV3/94 | Luciferase reporter assay/Western blotting/qRT-PCR | (105) |
| miR-584 | Down | LPIN1 | In vitro, human | PEO1, SKOV3, A2780, 3AO, CAOV3, OVCAR3/31 | Luciferase reporter assay/Western blotting/qRT-PCR | (106) |
| miR-4429 | Down | YOD1 | In vitro, human | OVCAR3, PEO1, A2780, 3AO, CAOV3, SKOV3/58 | Luciferase reporter assay/Western blotting/qRT-PCR | (107) |
| miR-200a-3p | Up | PCDH9 | In vitro, human | HO8919PM, ES2, HO8910, SKOV3/50 | Luciferase reporter assay/Western blotting/qRT-PCR | (98) |
| miR-874-3p/5p | Down | SIK2 | In vitro, human | Caov3, SKOV3/21 | Luciferase reporter assay/Western blotting/qRT-PCR | (108) |
| miR-532-5p | Down | TWIST1 | In vitro, human | SKOV3, OVCAR3, ES-2 CAOV-3/145 | Luciferase reporter assay/Western blotting/qRT-PCR | (109) |
| miR-132 | Down in SKOV3/DDP than SKOV3 | Bmi-1 | In vitro | SKOV3, SKOV3/DDP | Luciferase reporter assay/Western blotting/qRT-PCR | (110) |
| miR-552 | Up | PTEN | In vitro, human | HO8910, HGSOC/80 | Luciferase reporter assay/Western blotting/qRT-PCR | (111) |
| miR-125a-5p | Down | LIN28B | In vitro | A2780, SKOV3 | Luciferase reporter assay/Western blotting/qRT-PCR | (112) |
| miR-3173-3p | Up | NF90 | In vitro, in vivo, human | HeLa, 293T, A2780, SKOV3, HO8910, COV504, ES2, OVCAR3/169 | Luciferase reporter assay/Western blotting/qRT-PCR/RT-PCR | (79) |