Table 2.
Metastasis-related miRNAs in endometrial cancer.
| miRNA | Expression status (up/down) |
Targets | Model (in vitro, in vivo, human) | Cell lines/patient number | Target validation | References |
|---|---|---|---|---|---|---|
| miR-576-5p | Up | ZBTB4 | In vitro, in vivo | AN3-CA, Ishikawa, HEK-293 T | Western blotting/qRT-PCR | (134) |
| miR-501 | Up | HOXD10 | In vitro, human | AN3 CA, Ishikawa, HEK293T/33 | qRT-PCR | (135) |
| miR-449a | Down | NDRG1 | In vitro, in vivo, human | HEC-1A, AN3CA, KLE/87, HEC-1B | Luciferase reporter assay/Western blotting/qRT-PCR | (136) |
| miR-652 | Up | RORA | In vitro, in vivo, human | AN3 CA, RL95-2, Ishikawa, HEC-1-A, HEK293T/74, SPEC-2 | Luciferase reporter assay/Western blotting/qRT-PCR | (137) |
| miR-1271 | Down | LDHA | In vitro, human | ECC-1, KLE, AN3CA/30 | Luciferase reporter assay/Western blotting/qRT-PCR | (138) |
| miR-449a | Down | SRC | In vitro, human | KLE, AN3CA, HEC-1A, Ishikawa/40 | Luciferase reporter assay/Western blotting/qRT-PCR | (133) |
| miR-93-5p | Up | IFNAR1 | In vitro, human | Ishikawa, HEC-1B/50 | Western blotting/qRT-PCR | (139) |
| miR-218 | Down | ADD2 | In vitro, human | ECC-1, Ishikawa, KLE, AN3CA/25 | Luciferase reporter assay/Western blotting/qRT-PCR | (140) |
| miR-27b-3p | Down | MARCH7 | In vitro, in vivo, human | HEC-1-A, AN3CA, Ishikawa, RL95-2/66 | Luciferase reporter assay/Western blotting/qRT-PCR/immunohistochemistry | (128) |
| miR-101 | Down | COX-2 | In vitro, in vivo, human | AN3 CA, HEC-1-A/15 | Luciferase reporter assay/Western blotting/qRT-PCR/ELISA | (141) |
| miR-940 | Up | MRVI1 | In vitro, in silico | RL95-2, ISK, KLE | Luciferase reporter assay/Western blotting/qRT-PCR | (142) |
| miR-837 | Down | HDGF | In vitro, human | HEC-59, HEC-1B, AN3CA, KLE/47 | Luciferase reporter assay/Western blotting/qRT-PCR | (143) |
| miR-142 | Down | CCND1 | In vitro, in vivo, human | Ishikawa (ISK) HEC-1A/49 | Luciferase reporter assay/Western blotting/qRT-PCR | (144) |
| miR-139-5p | Down | HOXA10 | In vitro, human | Ishikawa, ECC1/25 | Luciferase reporter assay/Western blotting/qRT-PCR | (145) |
| miR-543 | Down | FAK, TWIST1 | In vitro, human | ECC-1, RL95-2AN3 CA/24 | Luciferase reporter assay/Western blotting/qRT-PCR | (146) |
| miR-30c | Down | MTA1 | In vitro, in vivo, human | HEC-1B, RL-952, Ishikawa/161 | Luciferase reporter assay/Western blotting/qRT-PCR/immunofluorescence staining | (147) |
| miR-202 | Down | FGF2 | In vitro, human | HEC-1-B, HEC-1-A/76 | Luciferase reporter assay/Western blotting/qRT-PCR | (148) |
| miR-381 | Down | IGF-1R | In vitro, human | HEC-1B, HEC-59, KLE, AN3CA/45, | Luciferase reporter assay/Western blotting/qRT-PCR | (149) |
| miR-214-3p | Down | TWIST1 | In vitro, human | HEC-1-A, HEC-1-B, RL95-2/22 | Luciferase reporter assay/Western blotting/qRT-PCR | (150) |
| miR-195 | Down | GPER | In vitro | AN3-CA, Hec1A | Luciferase reporter assay/Western blotting/qRT-PCR | (151) |
| miR-20a-5p | Down | STAT3 | In vitro, human | ECC-1, Ishikawa/41 | Luciferase reporter assay/Western blotting/qRT-PCR/RNA immunoprecipitation assay | (152) |
| miR-589-5p | Down | TRIP6 | In vitro, human | HEC-1B, AN3CA/40 | Luciferase reporter assay/Western blotting/qRT-PCR | (153) |
| miR-107-5p | Up | ERα | In vitro, in vivo, human | Ishikawa/71, HEC-1B | Luciferase reporter assay/Western blotting/RT-PCR/immunohistochemistry | (154) |
| miR- 34a, miR-424, miR-513 | Down | MMSET | In vitro, in vivo, human | HEC-1, Ishikawa/50 | Luciferase reporter assay/Western blotting/qRT-PCR | (155) |
| miR-206 | Down | HDAC6 | In vitro, human | Ishikawa, AN3C, RL95/44 | Luciferase reporter assay/Western blotting/qRT-PCR/RNA-seq | (82) |
| miR-320a, miR-340-5p | Down | eIF4E | In vitro, human | HEC-1A, Ishikawa, RL95-2/8 | Western blotting/qRT-PCR | (122) |
| miR-302a-5p, miR-367-3p | Down | HMGA2 | In vitro, in vivo, human | Ishikawa/40, HEC-1A | Luciferase reporter assay/Western blotting/qRT-PCR | (120) |
| miR-195 | Down | SOX4 | In vitro, human | KLE, RL95-2, HEC-1A, Ishikawa, 293T, hEEC/30 | Luciferase reporter assay/Western blotting/qRT-PCR | (156) |
| miR-200a | Up | FOXA2 | in vitro | RL95-2 | Luciferase reporter assay/Western blotting/qRT-PCR | (157) |
| miR-194 | Up | Sox3 | In vitro, in vivo, human | 19 EAC samples, Tumorspheres, stem cells |
Luciferase reporter assay/Western blotting/qRT-PCR | (158) |