TABLE 1.
Characteristics of DNA-based and RNA-based NAATs for CT, NG, and UU a
| Specimens | Claimed LOD | Template input | Total reaction vol | Total PCR cycle no. | |
|---|---|---|---|---|---|
| DNA-based NAATs | |||||
| CT detection kits | |||||
| Sansure | urethral swab, cervical swab | 400 copies/mL | 5 µL | 50 µL | 45 |
| Tianlong | urethral swab, cervical swab | 500 copies/mL | 4 µL | 40 µL | 40 |
| Liferiver | urethra and genital tract secretions | 1,000 copies/mL | 4 µL | 40 µL | 40 |
| Acon | urethral swab, cervical swab | 500 copies/mL | 4 µL | 40 µL | 40 |
| Bioperfectus | urethral swab, cervical swab | 500 IFU/mL | 5 µL | 25 µL | 40 |
| Daan | genitourinary secretions | 10 CT/mL | 2 µL | 45 µL | 40 |
| Fosun | genitourinary tract swab |
100 bacteria/mL | 4 µL | 30 µL | 40 |
| NG detection kits | |||||
| Sansure | urethral swab, cervical swab | 400 copies/mL | 5 µL | 50 µL | 45 |
| Tianlong | urethral swab, cervical swab | 500 copies/mL | 4 µL | 40 µL | 40 |
| Liferiver | urethra and genital tract secretions | 1,000 copies/mL | 4 µL | 40 µL | 40 |
| Acon | urethral swab, cervical swab | 500 copies/mL | 4 µL | 40 µL | 40 |
| Bioperfectus | urethral swab, cervical swab | 1 × 104 bacteria/mL | 5 µL | 25 µL | 40 |
| Daan | genitourinary secretions | 1.1 × 104 copies/mL | 2 µL | 45 µL | 40 |
| Fosun | genitourinary tract swab |
1 × 103 bacteria/mL | 4 µL | 30 µL | 40 |
| UU detection kits | |||||
| Sansure | urethral swab, cervical swab | 400 copies/mL | 5 µL | 50 µL | 45 |
| Tianlong | urethral swab, cervical swab | 500 copies/mL | 4 µL | 40 µL | 40 |
| Liferiver | urethra and genital tract secretions | 1,000 copies/mL | 4 µL | 40 µL | 40 |
| Acon | urethral swab, cervical swab | 500 copies/mL | 4 µL | 40 µL | 40 |
| Bioperfectus | urethral swab, cervical swab | 1 × 104 CCU/mL | 5 µL | 25 µL | 40 |
| Daan | genitourinary secretions | 1 × 104 copies/mL | 2 µL | 45 µL | 40 |
| Fosun | genitourinary tract swab | 100 CCU/mL | 4 µL | 30 µL | 40 |
| RNA-based NAATs (Rendu) | |||||
| CT detection kit | urethral swab, cervical swab, urine | 1,000 copies/reaction | 30 µL | 40 µL | 40 |
| NG detection kit | urethral swab, cervical swab, urine | 1,000 copies/reaction | 30 µL | 40 µL | 40 |
| UU detection kit | urethral swab, cervical swab, urine | 50 CFU/reaction | 30 µL | 40 µL | 40 |
a
NAAT, nucleic acid amplification test; CT, Chlamydia trachomatis; NG, Neisseria gonorrhoeae; UU, Ureaplasma urealyticum; and LOD, the limit of detection.