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. 2023 Aug 30;11(5):e01123-23. doi: 10.1128/spectrum.01123-23

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Copyright © 2023 Chen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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Fig 3 — CSYi-targeted repression of MexAB-OprM abolishes the elevated levofloxacin resistance in ΔczcR. (A) Relative expression of the mexAB-oprM genes in the ΔczcR strains containing the pCsy-MexA plasmid (mexA-i) or the control plasmid (Ctrl), which did not carry mini-CRISPR. The mexG gene was selected as a negative control. (B) Relative expression of the mexGHI-opmD genes in the ΔczcR strains containing the pCsy-MexG plasmid (mexG-i) or the control plasmid (Ctrl), which did not carry mini-CRISPR. The mexA gene was selected as a negative control. (C) Growth of the PAO1 WT and ΔczcR strains containing the pCsy-MexA (mexA-i) plasmid, the pCsy-MexA2 (mexA-i2) plasmid, or the control plasmid (Ctrl), which did not carry mini-CRISPR in the presence of levofloxacin. (D) Growth of the PAO1 WT and ΔczcR strains containing the pCsy-MexG (mexG-i) plasmid, the pCsy-MexG2 (mexG-i2) plasmid, or the control plasmid (Ctrl), which did not carry mini-CRISPR in the presence of levofloxacin. (E) Relative expression of the mexAB-oprM genes in the ΔczcR strains containing the pCsy-MexA2 plasmid (mexA-i2) or the control plasmid (Ctrl), which did not carry mini-CRISPR. The mexG gene was selected as a negative control. (F) Relative expression of the mexGHI-opmD genes in the ΔczcR strains containing the pCsy-MexG2 plasmid (mexG-i2) or the control plasmid (Ctrl), which did not carry mini-CRISPR. The mexA gene was selected as a negative control. Statistical significance is calculated based on the Student’s t-test (n.s., not significant; *P < 0.05; **P < 0.01; ***P < 0.001).