TABLE 3.
Activity profiles of several protein synthesis inhibitors in cell-free systems from sordarin-sensitive and -resistant Candida species
| Compound (concn [μg/ml]) | % Protein synthesis activity
|
Step inhibiteda | ||
|---|---|---|---|---|
| C. albicans | C. krusei | C. parapsilosis | ||
| Cycloheximide (1) | 94 | 15 | 25 | Translocation |
| Verrucarin A (1) | 5 | 8 | 7 | Peptide bond formation |
| Paromomycin (100) | 20 | 43 | 24 | Translocation, peptide bond formation |
| Anisomycin (1) | 41 | 50 | 36 | Peptide bond formation |
| Hygromycin (10) | 51 | 90 | 67 | Translocation |
| Puromycin (10) | 48 | 18 | 16 | Peptide bond formation |
| Emetine (100) | 77 | 97 | 74 | Unknown |
| Fusidic acid (100) | 77 | 100 | 80 | Translocation |
| Homoharringtonine (100) | 23 | 6 | 15 | Loading of aminoacyl-tRNA |
| Sordarin (1) | 1 | 100 | 94 | ? |
a
As described previously (2).