. 2023 Sep 21;11(5):e01651-23. doi: 10.1128/spectrum.01651-23

TABLE 1.

Plasmid profile and whole genome sequencing (WGS) outcome of bla _NDM-1-harboring plasmids in clinical P. rettgeri isolates, including S1-PFGE results and resistance gene content ^c

Isolate	Chromosome	Plasmid	Estimated size (kb) (S1-PFGE)	Size (bp) (WGS)	%GC	CDS	Inc-type (WGS)	AMR genes
PreM15628	chr ^d	–	–	4,368,530	40.81	4581	–	dfrA1
		p15628A_320	320	319,669	45.38	385	Col3M	arr-3, aac(6')-Ib-cr5, aph(3'')-Ib, aph(3')-Ia, aph(3')-VI, aph(6)-Id, bla _NDM-1 , bla _OXA-1 , bla _PER-2 , catB3, floR, mph(E), msr(E), qnrD1, sul1, sul2, tet(A)
		p15628B_125	142	125,717	43.52	149	Not typable
PreM15758	chr	–	–	4,368,564	40.81	4568	–	dfrA1
		p15758A_312^a	312	–	–	–	–	–
		p15758B_210	210	209,862	42.25	253	Col3M	arr-3, aac(6')-Ib-cr5, aph(3')-Ia, aph(3')-VI, bla _NDM-1 , bla _OXA-1 , catB3, qnrD1, sul1
		p15758C_98 ^b	– ^b	97,908 ^b	52.22	116	Not typable	aph(3'')-Ib, aph(6)-Id, floR, mph(E), msr(E), sul2, tet(A)
		p15758D_70	79	69,881	43.14	72	Not typable
		p15758E_55	62	55,812	44.01	81	Not typable
PreM15793	chr	–	–	4,367,913	40.47	4492	–	dfrA1
		p15793A_225	225 ^a	224,540	42.33	272	Col3M	arr-3, aac(6')-Ib-cr5, aph(3')-Ia, aph(3')-VI,bla _NDM-1 , bla_OXA-1, bla_PER-2, catB3, qnrD1, sul1
		p15793B_69	79	69,153	43.42	79	Not typable
		p15793C_57		57,581	34.66	92	Not typable

^{^a}

This plasmid was only seen in the S1-PFGE gel and hybridized with bla _NDM probe, but it was not found by whole genome sequencing (WGS).

^{^b}

The sequence of this plasmid was obtained by hybrid sequencing, but it was not observed in the S1-PFGE/Southern blot (Fig. S2).

^{^c}

Numbers in bold denote an estimated band size that gave positive signal after hybridization with bla _NDM-1 probe in S1-PFGE/Southern blot (Fig. S2).

^{^d}

chr, chromosome; –, not applicable.