TABLE 7.
Plasmids used in this study
| Plasmid | Construction | Replication origin | Antibiotic resistance marker | Host vector |
|---|---|---|---|---|
| pIL910 | USR 1500 bp of dgc2::KmR::DSR 1500 bp of dgc2 | ColE1 | ApR, KmR | pBlueScript SK II (+) |
| pIL985 | Sph I-dgc2-BamH I | ColE1 | CmR | pPBHLI18 |
| pIL1007 | Sph I-USR400 bp of dgc2::dgc2-BamH I | ColE1 | CmR | pIL985 |
| pIL1018 | Sph I-USR400 bp of dgc2::dgc2[AA]-BamH I | ColE1 | CmR | pIL1007 |
| pIL1019 | Sph I-USR400 bp of dgc2::dgc2[∆cat]-BamH I | ColE1 | CmR | pIL1007 |
| pIL1195 | USR1500 bp of pde1::KmR ::lacIq::Ptrc::LBDG_36750 (pde1)::DSR 1500 bp of pde1 | ColE1 | ApR, KmR | pBR322 |
| pIL1205 | LBDG21990_KmR ::lacIq::Ptrc::yhjH::LBDG_22000 | ColE1 | ApR, KmR | pIL1195 |