TABLE 1.
Drug susceptibility profile of amikacin-kanamycin-resistant laboratory strains and clinical isolates of M. tuberculosis and their mutations in the rrs gene
| Laboratory strain and clinical isolate of M. tuberculosisa | Aminoglycoside susceptibility (MIC in μg/ml)
|
Drug susceptibility profileb
|
Mutation in the rrs genec | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AK | KM | SM | INH | RIF | EMB | PZA | ETH | CIP | CAP | CYC | ||
| H37Rv (wild type) | 1 | 1 | 1 | S | S | S | S | NA | S | NA | NA | None |
| ATCC35827 (in vitro KMr mutant) | >128 | >128 | 8 | S | S | S | S | NA | S | NA | NA | A1400G |
| A2B (in vitro AKr mutant) | 128 | >128 | 4 | S | S | S | S | NA | S | NA | NA | None |
| A4B (in vitro AKr mutant) | 64 | 128 | 4 | S | S | S | S | NA | S | NA | NA | None |
| TN758 (P) | 8 | 4 | R | R | R | S | NA | R | S | R | R | None |
| TN933 (CB) | 8 | 4 | R | R | R | R | NA | NA | NA | NA | NA | None |
| TN2793 (C) | 32 | 32 | R | R | R | R | R | R | S | R | R | None |
| TN718 (AY) | 64 | 32 | R | R | R | R | R | R | S | S | S | None |
| TN4728 (BN) | >256 | >256 | R | R | S | S | NA | S | S | R | S | A1400G |
| TN810 (001/19) | >256 | >256 | R | R | R | R | R | S | S | S | S | A1400G |
| TN1521 (BU) | >256 | >256 | R | R | R | R | NA | S | S | S | S | A1400G |
| TN2229 (N2) | >256 | >256 | R | R | R | R | NA | S | S | R | S | A1400G |
| TN2508 (001/16) | >256 | >256 | R | R | R | R | NA | S | S | S | S | A1400G |
| TN4826 (H) | >256 | >256 | R | R | R | R | NA | S | S | R | S | A1400G |
| TN3360 (W) | >256 | >256 | R | R | R | R | NA | S | S | S | S | A1400G |
| TN3432 (W) | >256 | >256 | R | R | R | R | NA | R | R | R | S | A1400G |
| TN3187 (W1) | >256 | >256 | R | R | R | R | S | R | S | S | S | A1400G |
| TN3605 (W1) | >256 | >256 | R | R | R | R | NA | R | R | R | S | A1400G |
| TN3806 (W1) | >256 | >256 | R | R | R | R | NA | S | NA | NA | NA | A1400G |
| TN3218 (W3) | >256 | >256 | R | R | R | R | NA | S | S | S | S | A1400G |
| TN4800 (W33) | >256 | >256 | R | R | R | R | NA | R | S | R | S | A1400G |
a
Clinical isolates are indicated by the prefix TN and have the genotyped strain designation in parentheses.
b
INH, isoniazid; RIF, rifampin; EMB, ethambutol; PZA, pyrazinamide; ETH, ethionamide; CIP, ciprofloxacin; CAP, capreomycin; CYC, cycloserine; S, susceptible; R, resistant; NA, not available. The drug concentrations tested were as described in the text.
c
The entire rrs gene of H37Rv and the in vitro mutants were sequenced. Only region 1119 to 1536 of the rrs gene of the clinical isolates was sequenced. The numbering of base 1400 refers to M. tuberculosis (11).