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pkd1l1 knockout alters hepatobiliary function. (A) Representative images of PED6 uptake in larvae showing typical normal (top), faint (middle) and absent (bottom) fluorescence (red circles) in gallbladders. Scale bars: 500 µm. (B) Quantification of PED6 uptake showed an increase number of absent gallbladders in pkd1l1 mutant (n=145) compared to wild-type (n=84) larvae (***P<0.001; χ2 test), reflecting defects in biliary function.
