Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Caenorhabditis elegans) | N2 | Caenorhabditis Genetics Center (CGC) | RRID:WB-STRAIN:WBStrain00000003 | Wild-type (Bristol) |
| Strain, strain background (Caenorhabditis elegans) | CL2070 | CGC | RRID:WB-STRAIN:WBStrain00005096 | dvIs70 [hsp-16.2p::GFP+rol-6(su1006)] |
| Strain, strain background (Caenorhabditis elegans) | AM140 | CGC | RRID:WB-STRAIN:WBStrain00000182 | rmIs132Punc-54::q35::yfp |
| Strain, strain background (Caenorhabditis elegans) | KX54 | CGC | RRID:WB-STRAIN:WBStrain00024080 | ifg-1(cxTi9279) |
| Strain, strain background (Caenorhabditis elegans) | PS3551 | CGC | RRID:WB-STRAIN:WBStrain00007673 | hsf-1(sy441) |
| Genetic reagent (Caenorhabditis elegans) | myo3p::GFP-IRES-tdTomato | This paper | Adapted from: DOI: 10.2144/000113821 See Materials and methods, Method for making bi-cistronic vector |
|
| Antibody | Anti-puromycin (Mouse monoclonal) | MilliporeSigma | Cat#: MABE343 RRID:AB_2566826 |
1:5000 |
| Antibody | Anti-GAPDH (Rabbit polyclonal) |
Proteintech | Cat#: 1094-1-AP RRID:AB_2263076 |
1:5000 |
| Antibody | Anti-myc (Mouse Monoclonal) | Cell Signaling | Cat#: 2276S RRID:AB_331783 |
1:2000 |
| Antibody | Anti-mouse—HRP (secondary) | Cell Signaling | Cat#: 7076S, RRID:AB_330924 | 1:5000 |
| Antibody | Anti-rabbit—HRP (secondary) | Cell Signaling | Cat#: 7074S, RRID:AB_2099233 | 1:5000 |
| Commercial Assay or kit | 20S Proteasome Activity Assay Kit | Sigma-Aldrich | Cat#: APT280 | |
| Commercial Assay or kit | SYPRO Ruby Protein Gel Stain 1× | Bio-Rad | Cat#: 1703125 | |
| Chemical compound, drug | Anisomycin | MedChemExpress | Cat#: HY-18982 | |
| Chemical compound, drug | Lycorine hydrochloride | Combi-Blocks | Cat#: QW-2476 | |
| Chemical compound, drug | 4E1RCat | MedChemExpress | Cat#: HY-14427 | |
| Chemical compound, drug | Puromycin | Sigma-Aldrich | Cat#: P8833 | |
| Chemical compound, drug | Salubrinal | MedChemExpress | Cat#: HY-15486 | |
| Chemical compound, drug | Bortezomib, free base | LC Laboratories | Cat#: B-1408 |