Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Biological sample (Oryctolagus cuniniculus) | Gonads | Hypharm | NZ1777 | New Zealand rabbits |
| Commercial kit | RNAscope kit | ACD | 322310 | 2.5HD assay-brown |
| Sequence-based reagent | RNAscope probe anti-DMRT1 | ACD | 410481 | XM_002708188.1 |
| Sequence-based reagent | RNAscope probe anti-SRY | ACD | 803191 | AY785433.1 |
| Sequence-based reagent | RNAscope probe anti-POU5F1 | ACD | 513271 | NM_001099957.1 |
| Sequence-based reagent | RNAscope probe anti-RSPO1 | ACD | 488231 | XM_002720657.2 |
| Antibody | Anti-DMRT1 (mouse monoclonal) | Santa Cruz | sc-377167 | IHC (1:500) IF (1:200) WB (1:100) |
| Antibody | Anti-SOX9 (rabbit polyclonal) | Francis Poulat | IHC (1:500) IF (1:200) |
|
| Antibody | Anti-FOXL2 (rabbit polyclonal) | Boulanger et al., 2014 | IHC (1:500) | |
| Antibody | Anti-POU5F1 (goat polyclonal) | Santa Cruz | sc-8628 | IHC (1:500) IF (1:200) |
| Antibody | Anti-PAX8 (rabbit polyclonal) | Proteintech | 10226-1-AP | IHC (1:6000) |
| Antibody | Anti-Ki67 (rabbit monoclonal) | Thermo Scientific | MA5-14520 | IHC (1:500) |
| Sequence-based reagent | SRY_F | This paper | PCR primers | TGCTTACACACCAGCCAAACA |
| Sequence-based reagent | SRY_R | This paper | PCR primers | TTCCTGGCCGCTCACTTTAC |
| Sequence-based reagent | DMRT1_F | This paper | PCR primers | GGAGCCTCCCAGCACCTTA |
| Sequence-based reagent | DMRT1_R | This paper | PCR primers | TGCATCCTGTACTGCGAACTCA |
| Sequence-based reagent | SOX9_F | This paper | PCR primers | GGCTCCGACACCGAGAATACAC |
| Sequence-based reagent | SOX9_R | This paper | PCR primers | GAACTTGTCCTCTTCGCTCTCCTT |
| Sequence-based reagent | CYP19A1_F | This paper | PCR primers | GGAAGAATGCATCGACTTGAGTT |
| Sequence-based reagent | CYP19A1_R | This paper | PCR primers | GGGCCCAAAACCAAATGGT |
| Sequence-based reagent | RSPO1_F | This paper | PCR primers | GCCCGCCTGGATACTTCGA |
| Sequence-based reagent | RSPO1_R | This paper | PCR primers | GGTGCAGAAGTTGTGGCTGAA |
| Sequence-based reagent | FOXL2_F | This paper | PCR primers | TTTCCCCTTTCCCCCATCTG |
| Sequence-based reagent | FOXL2_R | This paper | PCR primers | CTGAACCTTGCACCCAGCAT |
| Sequence-based reagent | AMH_F | This paper | PCR primers | GCTCATCCCCGAGACCTAC |
| Sequence-based reagent | AMH_R | This paper | PCR primers | CATCTTCAACAGCAGCACC |
| Sequence-based reagent | DHH_F | This paper | PCR primers | GCAATAAGTACGGGCTGCTG |
| Sequence-based reagent | DHH_R | This paper | PCR primers | GGCCAGGGAGTTATCAGCTT |
| Software | qBase+ | Biogazelle | ||
| Software | GraphPad Prism | GraphPad Software |