| Genetic reagent (mouse) |
Id2-CreER |
Jax |
016222 |
|
| Genetic reagent (mouse) |
Dlx5/6-Flpe |
Jax |
010815 |
|
| Genetic reagent (mouse) |
NPY-hrGFP |
Jax |
006417 |
|
| Genetic reagent (mouse) |
Nkx2.1-Flpo |
Jax |
028577 |
|
| Genetic reagent (mouse) |
VIP-Flpo |
Jax |
028578 |
|
| Genetic reagent (mouse) |
Ai65 |
Jax |
021875 |
|
| Genetic reagent (mouse) |
Ai80 |
Jax |
025109 |
|
| Genetic reagent (mouse) |
FLTG |
Jax |
026932 |
|
| Antibody |
Rabbit polyclonal anti-CCK |
Frontier Institute |
Af350 |
1:500 dilution |
| Antibody |
Donkey polyclonal anti-rabbit AlexaFluor-488 |
Invitrogen |
A-21206 |
1:1000 dilution |
| Sequence-based reagent |
Cre primer (fwd) |
Invitrogen |
custom |
caacgagtgatgaggttcgca |
| Sequence-based reagent |
Cre primer (rev) |
Invitrogen |
custom |
cgccgcataaccagtgaaaca |
| Sequence-based reagent |
Flpe primer (fwd) |
Invitrogen |
custom |
tctttagcgcaaggggtagga |
| Sequence-based reagent |
Flpe primer (rev) |
Invitrogen |
custom |
aagcacgcttatcgctccaa |
| Sequence-based reagent |
Flpo primer (fwd) |
Invitrogen |
custom |
ccacattcatcaactgcggc |
| Sequence-based reagent |
Flpo primer (rev) |
Invitrogen |
custom |
gggccgttcttgatagcgaa |
| Sequence-based reagent |
NPY-hrGFP primer (fwd) |
Invitrogen |
custom |
atgtggacggggcagaagatc |
| Sequence-based reagent |
NPY-hrGFP primer (rev) |
Invitrogen |
custom |
gtgcggttgccgtactgga |
| Sequence-based reagent |
NPY cRNA antisense probe primer (fwd) |
Invitrogen |
custom |
tcacagaggcacccagagc |
| Sequence-based reagent |
NPY cRNA antisense probe primer (rev +T7) |
Invitrogen |
custom |
attaatacgactcactatag cggagtccagcctagtggtg |
| Sequence-based reagent |
tdTomato cRNA antisense probe primer (fwd) |
Invitrogen |
custom |
atcaaagagttcatgcgcttc |
| Sequence-based reagent |
tdTomato cRNA antisense probe primer (rev +T7) |
Invitrogen |
custom |
cattaatacgactcactataggg ttccacgatggtgtagtcctc |
| Chemical compound, drug |
tamoxifen |
Sigma |
T5648 |
Prepare 20 mg/ml stock in corn oil |