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. Author manuscript; available in PMC: 2023 Oct 17.
Published in final edited form as: Lab Chip. 2023 Apr 12;23(8):2005–2015. doi: 10.1039/d3lc00066d

Fig. 5.

Fig. 5

High-throughput UOMS-AST enabled by double-ELR and under-oil sweep distribution. (A) A schematic shows the physics of double-ELR and the operation of under-oil sweep distribution. (B) A camera picture shows an array of 520 under-oil spots (0.5 mm in diameter, 4 nL per spot) prepared by under-oil sweep distribution under silicone oil (20 cSt). The device is a 1-well chambered coverglass device (Nunc Lab-Tek II - borosilicate glass #1.5 - biocompatible acrylic adhesive, 6 cm × 2.4 cm, 0.16–0.19 mm in bottom thickness) compatible with silicone oil. The liquid is DMEM culture media + 10% fetal bovine serum (FBS) with 1 mM FITC for visualization. Dry spots were marked out with white circles. Different species and/or antibiotic type/concentration can be applied to the local domains on a device by sweeping a sample on a designated area. (C) A camera picture shows a home-made sample holder that houses 4 pieces of chambered coverglass in an onstage incubator on a scope.