Fig. 1.

Schematic of the Super-Res-Chip System. (a) Building blocks of the Chip: PDMS-ring with PC-membrane (left) and 3D-printed Chip (right); top- and side-view of the assembled platform. (b) Scheme of the co-culture in the chip: U87 glioblastoma cells cultured on the upper side, together with HUVEC cells on the bottom side of the membrane either directly on the membrane (top) or in a layer of Matrigel (approx. 150µm). (c) Principle of dSTORM imaging of the membrane: The PDMS ring is cut off, the membrane inserted into the Attofluor cell chamber in between two round glass coverslips, together with imaging buffer (top). Attofluor cell chamber for microscopy of the membranes (bottom). Schemes in (b) and (c) were created with BioRender.com.