Table 2.
Summary of bacterial reverse mutation assay results (pre-experiment I; up to 5,000 µg/plate)—mean ± SD revertant colonies.
| Tester strains | TA98 | TA100 | TA1535 | TA1537 | WP2 uvrA | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| ±S9 | −S9 | +S9 | −S9 | +S9 | −S9 | +S9 | −S9 | +S9 | −S9 | +S9 |
| Water | 37 ± 19.2 | 40 ± 3.8 | 104 ± 5.1 | 104 ± 1 | 13 ± 0.6 | 9 ± 3 | 11 ± 2.9 | 13 ± 1.7 | 254 ± 7 | 279 ± 23.6 |
| 31.6 µg/plate | 34 ± 2 | 37 ± 8.5 | 108 ± 4.9 | 105 ± 13.2 | 15 ± 2.1 | 6 ± 3.2 | 18 ± 1.7 | 13 ± 5 | 213 ± 36.1 | 242 ± 32.7 |
| 100 µg/plate | 33 ± 5.7 | 35 ± 4.9 | 92 ± 9.6 | 108 ± 7.2 | 9 ± 3.2 | 6 ± 1.2 | 11 ± 1.2 | 10 ± 1.7 | 228 ± 14.6 | 271 ± 15.6 |
| 316 µg/plate | 33 ± 7 | 37 ± 6.4 | 101 ± 17.1 | 95 ± 13.2 | 12 ± 1.5P | 5 ± 1P | 9 ± 3.1P | 10 ± 2.5P | 283 ± 1.85P | 299 ± 18.6P |
| 1000 µg/plate | 42 ± 10.5P | 42 ± 6.2P | 90 ± 8.7P | 108 ± 7.2P | 9 ± 2.9P | 9 ± 4.4P | 11 ± 1.2P | 13 ± 3.8P | 277 ± 26.9P | 300 ± 26.5P |
| 2500 µg/plate | 30 ± 10.4P | 44 ± 8.7P | 103 ± 1.7P | 98 ± 7.6P | 8 ± 1.2P | 8 ± 1.2P | 10 ± 2.5P | 14 ± 5.6P | 202 ± 2.6P | 238 ± 30.6P |
| 5000 µg/plate | 24 ± 5.8P | 45 ± 2.6P | 89 ± 10P | 93 ± 8.7P | 5 ± 3P | 6 ± 0.6P | 9 ± 3.1P | 9 ± 4.2P | 222 ± 7.6P | 265 ± 22.1P |
| 4-NOPD (10 µg/plate) | 542 ± 45 | NA | 432 ± 22 | NA | 918 ± 78.4 | NA | 60 ± 15.5 | NA | 1255 ± 61.2 | NA |
| 2-AA (2.5 µg/plate) | NA | 2057 ± 223.1 | NA | 1361 ± 19.2 | NA | 264 ± 10.1 | NA | 175 ± 11.9 | NA | 693 ± 125 |
2-AA = 2-aminoanthracene; LegH = soy leghemoglobin protein; NA = data not applicable; 4-NOPD = 4-nitro-o-phenylenediamine; P: visible precipitate; SD = standard deviation; +S9 = containing phenobarbital/B-naphthoflavone-induced Sprague–Dawley rat liver microsomal fraction; −S9 = without S9 microsomal fraction. Data are shown as mean ± SD revertants/plate for three replicates for each concentration in each experiment.