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. 2023 Jul 27;30(10):1403–1413. doi: 10.1038/s41417-023-00647-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A The levels of EMT markers were determined via IB analysis. B Detection of EMT markers by IB analysis was performed in HCT116 and DLD-1 cells transfected with siRNAs targeting cIAP1 (sicIAP1) or cIAP2 (sicIAP2) or with control siRNA (siCon). C Migration and invasion assays were performed with DLD-1 cells pre-treated with WNT3A for 48 h. Upper panels: Migration assay images (Left) and quantification graph (Right). Lower panels: invasion assay images (Left) and quantification graph (Right). D Migration assay with stable transfectant DLD-1 cell lines (DLD-1^con and DLD-1^shRIP1) treated with or without WNT3A for 48 h. Left panels: Migration assay images. Right panel: Quantification graph. E Invasion assay with DLD-1^con and DLD-1^shRIP1 treated with or without WNT3A for 48 h. Left panels: Invasion assay images. Right panel: quantification graph.