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. 2023 Sep 4;9(10):1697–1708. doi: 10.1038/s41477-023-01510-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, Loading assay showing that accumulation of [³H]-ABA is decreased in cells expressing ABCG25 compared with untransfected control cells. All data points represent five independent measurements. Data are mean ± s.d. b, Efflux assay showing that [³H]-ABA retention is decreased in cells expressing ABCG25 compared with control cells. Data points represent three independent measurements. Data are mean ± s.d. c, ATPase activities of ABCG25_WT and ABCG25_EQ in detergent micelle in the presence of different concentrations of ATP. Data points were nonlinear-fitted using the Michaelis–Menten equation. Data points represent four independent measurements. Data are mean ± s.d. d, ABA concentration-dependent ATPase activity of ABCG25_WT in detergent micelle. Data points were nonlinear-fitted using the allosteric sigmoidal model. Data points represent three independent measurements. NS, not significant; *P < 0.05 for ABA-added versus no ABA (one-way analysis of variance (ANOVA) with Dunnett’s multiple comparisons test). Data are mean ± s.d. e, ATPase activities of ABCG25_WT and ABCG25_EQ reconstituted into liposomes in the presence of different concentrations of ATP. Data points were nonlinear-fitted using the Michaelis–Menten equation. Data points represent four independent measurements. Data are mean ± s.d. f, ABA concentration-dependent ATPase activity of ABCG25_WT reconstituted into liposomes. Data points were nonlinear-fitted using the allosteric sigmoidal model. Data points represent three independent measurements. *P < 0.05 for ABA-added versus no ABA (one-way ANOVA with Dunnett’s multiple comparisons test). Data are mean ± s.d.

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