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. 2023 Sep 4;9(10):1697–1708. doi: 10.1038/s41477-023-01510-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — a, The EM densities interpreted as cholesterol molecules in the apo-state ABCG25_WT (DDM plus CHS extraction) map. The maps are prepared in three different contour levels using Coot. b, The EM densities interpreted as cholesterol molecules in the apo-state ABCG25_WT (DDM alone extraction) map. The maps are prepared in three different contour levels using Coot. c, The EM densities of ABA molecules in the ABA-bound ABCG25_WT (DDM plus CHS extraction) map. The maps are prepared in three different contour levels using Coot. d, The EM densities of ABA molecules in the ABA-bound ABCG25_WT (DDM extraction with ABA added) map. The maps are prepared in three different contour levels using Coot. e, A representative gel filtration and Coomassie-blue-staining SDS-PAGE result of wild-type ABCG25 purified with DDM alone extraction. Experiments have been repeated for at least three times with similar results. Uncropped gel can be found in Source Data. f, The gold-standard Fourier shell correlation curve for the overall map of the apo-state ABCG25_WT (DDM extraction). The resolution reaches 3.7 Å by a threshold of 0.143. g, The local resolution map of the apo-state ABCG25_WT (DDM extraction) calculated using ResMap. h, A representative gel filtration and Coomassie-blue-staining SDS-PAGE result of wild-type ABCG25 purified with DDM extraction and ABA-added during the whole purification steps. Experiments have been repeated for at least three times with similar results. Uncropped gel can be found in Source Data. i, The gold-standard Fourier shell correlation curve for the overall map of the ABA-bound ABCG25_WT (DDM extraction with ABA added). The resolution reaches 3.2 Å by a threshold of 0.143. j, The local resolution map of the ABA-bound ABCG25_WT (DDM extraction with ABA added) calculated using ResMap.

Source data