Extended Data Fig. 8. Cryo-EM analysis of ABCG25EQ in the ATP-bound state.
a, A representative gel filtration and Coomassie-blue-staining SDS-PAGE result of the E232Q mutant of ABCG25 (ABCG25EQ). Experiments have been repeated for at least three times with similar results. Uncropped gel can be found in Source Data. b, A typical cryo-EM image of the ATP-bound ABCG25EQ. Experiments have been repeated for at least three times with similar results. c, Typical 2D classification images of the ATP-bound ABCG25EQ. Experiments have been repeated for at least three times with similar results. d, The gold-standard Fourier shell correlation curve for the overall map of the ATP-bound ABCG25EQ. The resolution reaches 3.0 Å by a threshold of 0.143. e, Euler angle distribution of the ATP-bound ABCG25EQ. f, The local resolution map of the ATP-bound ABCG25EQ calculated using ResMap. g, A flowchart for the data processing of the ATP-bound ABCG25EQ. h, The EM densities for the six transmembrane helices, CnH, CpH and E-helix of the ATP-bound ABCG25EQ map. Residues with notable side chains are labeled aside. i, The EM densities of ATP and Mg2+ in the ATP-bound ABCG25EQ map.