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. 2023 Sep 4;9(10):1697–1708. doi: 10.1038/s41477-023-01510-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a, A flowchart for the data processing of ABCG25_WT with DDM plus CHS extraction in the apo-state. Details can be found in the ‘Image processing’ session in Methods. b, A flowchart for the data processing of ABCG25_WT (DDM plus CHS extraction) in the ABA-bound state. Details can be found in the ‘Image processing’ session in Methods. c, EM densities for the six transmembrane helices, CnH, CpH and E-helix of the apo-state ABCG25_WT (DDM plus CHS extraction) map. Residues with notable side chains are labeled aside.