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. 2023 Sep 28;9(10):1643–1658. doi: 10.1038/s41477-023-01526-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 9 — Eight loci were analyzed, each expressing two isoforms generated from alternative splicing event. Glutathione S-transferase (GST), Terpene synthase (TPS), FAD-dependent hydroxylase (Hpxo) ubiquitin-like-specific protease 2B (ULP2B), MYB1R, Universal phosphorylated stress protein (PHOS34), TBC1 and Fbox. For each locus, the top scheme of the panel show the distribution of exons (dark grey rectangles), skipped exons (light grey rectangles) and retained introns (light grey lines) for both isoform, as well as the percentage of unique circular consensus sequence (CCS) detected in the IsoSeq libraries. The corresponding functional domain(s) predicted using Pfam (pfam.xfam.org) is displayed below each isoform. qRT-PCR reactions were designed to selectively amplify only one isoform. The position of the amplicon is indicated for each isoform by an orange or yellow line. Their level of expression was normalized to the ACTIN housekeeping gene using the ∆Ct method.