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. 2023 Oct 17;13:114. doi: 10.1186/s13568-023-01617-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A) Enzyme digested products of shuttle vector pFastBac Dual (5238 bp) before cloning on 1% agarose gel. DNA ‎ladder 200 bp-10 kb (200, 400, 600, 800, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, 10,000; Thermo Science fisher, ‎Germany).‎ B) PCR products of four structural genes of NDV 2018‎‏/‏‎(VIId) Ck/IR/MAM strain on 1% agarose gel; M gene (1094 ‎bp), F gene (1661 bp), and HN gene (1716 bp), respectively, DNA ladder 200 bp-10 kb. C) Enzyme digested products of pFast (5238 bp) + GFP (717 bp) + P2A (54 bp) + F gene (1661 bp) + M gene (1094 bp) = 8764 ‎bp for cloning verification on 1% agarose gel; DNA ladder 200 bp-10 kb (Thermo Sciencefisher, Germany)