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. 2023 Oct 17;13:114. doi: 10.1186/s13568-023-01617-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Gating strategy for the flow cytometric analysis of mice T-cells, platelets, and their subpopulations. A single-cell suspension was stained with fluorochrome-conjugated antibodies against CD4-FL4- APC and CD8-FL1-488. Data were collected with a MACSQuant flow cytometer and analyzed with FlowJo Software. Lymphocytes were identified by their scatter properties (FSC-A × SSC-A plot) and doublets were excluded by gating on FSC-A × FSC-H. CD4 + or CD8 + cells represent T-cell subsets in immunized mice with (a) only NDV-VLPs and (b) NDV-VLPs boosted with B1 NDV live vaccine