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. 2023 Oct 17;14:6543. doi: 10.1038/s41467-023-42085-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b A tomographic reconstruction of the plasma-FIB milled HeLa cells data set containing 80 S ribosomes (Berger et al, 2023), EMPIAR-11306. The tomogram in a is reconstructed from a tilt stack aligned by patch tracking in IMOD a, and post-processed with IsoNet b. c The resulting 80 S ribosome structure at the resolution 6.2 Å sliced in the middle of the reconstruction and colored according to local resolution. Black scale bar: 10 nm. Lower panel: estimation of the resolution based on Fourier Shell Correlation between independently refined half-sets.