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. 2023 Oct 17;14:6531. doi: 10.1038/s41467-023-42188-4

Fig. 1. The expression and regulation of renal adiponectin.

Fig. 1

Determination of adiponectin expression levels, regulation and location of adiponectin expression in kidney cells using wildtype, glucagon receptor KO, liver specific Pck1 KO and kidney-specific PPARγ KO mice. A Adiponectin expression in Epididymal adipose tissue (Epi) (n = 5), kidney (n = 4), spleen (n = 5), liver (n = 5), intestine (n = 5), brain (n = 5) and heart (n = 5). B The kidney weight as a percentage of body weight in wild type (WT) (n = 23), adiponectin knockout (AKO) (n = 25) and delta-Gly adiponectin overexpression (dGly) mice (n = 19). P values were determined by Tukey’s multiple comparison test. C The kidney weight as a percentage of body weight before and after unilateral nephrectomy (UNx) (WT n = 5, AKO n = 8) P values were determined by Sidak’s multiple comparison test. D The expressions of ADIPOQ and GCGR in human kidney biopsy samples from Gene Expression Omnibus (n = 60). E Adiponectin expression in the cortex and medulla in the kidney (Cortex n = 10, medulla n = 8). F Adiponectin expression in the whole kidney after 1 week cold exposure under fed conditions (Control n = 4, cold exposure n = 5). G Adiponectin expression in the whole kidney after 3 days cold exposure and 16-hour fasting (Control and cold fasting n = 12). H Adiponectin expression in the kidney of global glucagon receptor KO mice (Control n = 10, Glucagon R KO n = 12). I Adiponectin expression in the kidney of liver-specific Pck1 KO mice (Albumin-Cre/Pck1 flox mice) (Control n = 12, Liver pck1 KO n = 8) J Adiponectin expression in the kidney of kidney-specific PPARγ KO mice (Six2-Cre/PPARγ flox mice) (Control n = 11, Kidney PPARγ KO n = 7). K Single cell-seq reads were mapped to the reference genome. Visualization of adiponectin expression using IGV. Abbreviations are as follows. NP nephron progenitors; Podo podocyte; PCT proximal convoluted tubule; PST proximal straight tubule; LOH ascending loop of Henle; CNT connecting tubule; DCT distal convoluted tubule; PC collecting duct principal cell; IC collecting duct intercalated cell; Endo containing endothelial, vascular, and descending loop of Henle; Immune cells, including macrophages, neutrophils, lymphocytes; Stroma, stromal cell. L Scatter plot representation of adiponectin expression in each kidney cell type in adult kidney and postnatal day (P)0 (P0) kidneys. M ATAC-seq reads mapped to the adiponectin locus was visualized by IGV software. ATAC-seq was performed in duplicate utilizing whole kidney of P0, P21 and P56. Data are mean ± SEM. Unpaired two-tailed student t-tests were performed from E to J.