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. 2023 Oct 17;14:6531. doi: 10.1038/s41467-023-42188-4

Fig. 3. Renal adiponectin overexpression mice exhibit an enhanced gluconeogenic phenotype.

Fig. 3

A Validation of adiponectin overexpression in the cortex and the medulla of the kidney after 1week doxycycline treatment (600 mg per kg diet weight) (Control Cortex n = 3, KSPAPN cortex n = 3, KSPAPN medulla n = 3, Control Medulla n = 4). B Serum adiponectin levels after 1week doxycycline treatment (Control n = 6, KSPAPN: n = 11). C Representative western blot of adiponectin (top) and α-tubulin (bottom) (n = 3). D Schematic illustration of the time course of the experiment. Doxycycline 600 mg/kg containing (dox600) HFD was started at the age of 7–10 weeks old. Tolerance tests were performed after 2months of HFD dox600. Tissues were harvested after 10 months of HFD feeding. E Bodyweight after 10 months of HFD dox600 (Control n = 6, KSPAPN n = 8). F Body composition measured by echo nuclear magnetic resonance (echo MRI) (Control n = 6, KSPAPN: n = 8). G Tissue weight after 10 months of HFD dox600 (Control n = 5, KSPAPN: n = 10). H Blood Glucose levels at different time points in OGTT (Control n = 5, KSPAPN: n = 10). I Blood insulin level at different time points in OGTT (Control n = 5, KSPAPN: n = 10). J Blood glucose level at different time points after insulin injection (Control n = 16, KSPAPN: n = 20). K Blood glucose levels at different time points after 3HB injection (Control n = 10, KSPAPN n = 7). L Blood glucose level at different time points after Glutamine injection (Control n = 6, KSPAPN n = 11). M Blood glucose levels at different time points after glycerol injection (Control n = 6, KSPAPN n = 11). N Blood glucose levels at different time points after pyruvate gavage (Control n = 8, KSPAPN n = 7). O Blood glucose levels (fold-change) at different time points after alanine injection (Control n = 14, KSPAPN n = 10). P Glucose production rate per kidney during the perfusion of kidneys with pyruvate-containing perfusate (n = 3). Q Gene expression of fibrosis markers (Control n = 9, KSPAPN n = 13). R Representative trichrome staining images of the kidney. (Scale bar: 50μm) S Gene expression of lipogenesis markers (Control n = 9, KSPAPN n = 13). T Gene expression of lipid uptake markers (Control n = 8, KSPAPN n = 13). Data are mean ± SEM. 2way ANOVA with 2-stage linear step-up procedure of BKY correction for multiple comparisons was performed to determine p-value from (H) to (P). Multiple unpaired two-tailed t-tests with 2-stage linear step-up procedure of BKY correction for multiple comparisons were utilized to determine p-values for (Q), (S) and (T).