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. 2023 Oct 17;13:17648. doi: 10.1038/s41598-023-42178-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Independent depletion of each common OST subunit suppresses LOX N-glycosylation and OST48 expression. Western blots for LOX, OST48 and vinculin (loading control) 12 days after CRISPR/Cas9-mediated genome targeting for the indicated OST genes in U87 cells. Samples were re-seeded 10 days after transduction and moved to normoxia (N) or hypoxia (H; 1% O₂) on day 11 for 24 h.