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. 2023 Oct 17;14:6548. doi: 10.1038/s41467-023-41890-7

Fig. 5. In vivo evaluation of an activation loop mutant of c-Src in Drosophila.

Fig. 5

a Expression Src42A WT and Y400F mutant in the Drosophila eye using a GMR-Gal4 promoter. Representative images of eyes from GMR-Gal4 (control), GMR-Gal4-Src42A WT and GMR-Gal4-Src42A Y400F flies. Scale bar, 500 μm. b Expression of Src42A WT and Y400F in the Drosophila wing using a salEPv-Gal4 promoter. Representative images of wings from salEPv-Gal4 (control), salEPv-Gal4-Src42A and salEPv-Gal4-Src42A Y400F flies. Scale bar, 500 μm. For (a) and (b), data shown are representative of ≥10 images. c Expression of Src42A WT and Src42A Y400F in the developing Drosophila tracheal tube using the btl-Gal4 promoter. Images show projections of confocal sections of lateral views of stage 16 embryos stained for CBP to visualize the tracheal tubes. The length of the DT (arrow in control) is quantified in (e). Scale bar, 100 μm. d Rescue experiments to assay Src42A Y400F function. Projections of confocal sections of lateral views of stage 16 embryos stained for CBP to visualize the tracheal tubes. Src mutant embryos (SrcF80 and SrcKM) show reduced DT length. Scale bar, 100 μm. Expression of Src42A Y400F in the trachea of SrcF80 mutants restores DT length (quantifications are shown in (e)), control (n = 19), btl>Src (n = 9), btl>Src Y400F (n = 15), btl>SrcKM (n = 6), SrcF80 (n = 6), SrcF80; btl>Src Y400F (n = 9). Unpaired two-tailed Student’s t-test applying Welch’s correction. Differences were considered significant when p < 0.05. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 where n.s. means not statistically significant. Source data are provided in the Source Data file.