TABLE 1.
Collaboration of VCZ or AmB with PMN for A. fumigatus hyphal growth inhibition: MTT assay
| Condition | A570 (n = 4)a | % Inhi- bition |
Pb
|
||
|---|---|---|---|---|---|
| Ic | IId | IIIe | |||
| Medium (control) | 0.539 ± 0.128 | 0 | R | ||
| VCZ (0.5 μg/ml) | 0.319 ± 0.105 | 41 | <0.05 | ||
| PMN (2 × 105/well) | 0.381 ± 0.114 | 30 | NS | R | |
| PMN + VCZ | 0.137 ± 0.010 | 75 | <0.01 | <0.01f | |
| AmB (0.5 μg/ml) | 0.142 ± 0.030 | 74 | <0.01 | R | |
| PMN + AmB | 0.228 ± 0.050 | 58 | <0.01 | <0.01 | NS |
a
MTT metabolism by hyphae (isolate 92-270) after exposure to VCZ, AmB, PMN plus VCZ, or PMN plus AmB for 24 h at 37°C (105 germlings/well). Values are means ± standard deviations.
b
R, reference value; NS, not significant (P > 0.05).
c
Metabolism of MTT in medium (control) cultures compared to MTT metabolism in test cultures.
d
MTT metabolism by hyphae from PMN cultures compared to that by hyphae from PMN plus VCZ or PMN plus AmB cultures.
e
MTT metabolism by hyphae from AmB cultures compared to that by hyphae from PMN plus AmB cultures.
f
PMN plus VCZ was also significant (P < 0.01) compared to VCZ alone.