FIGURE 3.

Fluorescence traces of tgTas2r1 expressing cells stimulated with bitter activators. The tgTas2r1 expression construct was transiently transfected in HEK 293T-Gα16gust44 cells and challenged with 72 compounds of a bitter compound library. The fluorescence traces of compounds eliciting responses (blue traces) are shown together with the corresponding substance concentrations. The traces were averaged from one representative experiment performed in duplicate wells. Empty vector transfected and identically treated cells were used as negative controls (gray traces). Scale bar, bottom right.