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. 2023 Oct 4;14:1213052. doi: 10.3389/fphar.2023.1213052

TABLE 2.

Solvent combinations and Rf value of column eluents of ethyl acetate fraction of O. sanctum.

Tracks Solvent combination Rf Tracks Solvent combination Rf
01 Gallic acid 0.52 20 Toluene + 25% ethyl acetate-1 0.62
02 Ellagic acid 0.55 21 Toluene + 25% ethyl acetate-2 0.52, 0.63, 0.74
03 Kaempferol 0.81 22 Toluene + 25% ethyl acetate-3 0.54
04 Quercetin 0.73 23 Toluene + 50% ethyl acetate-1 0.34, 0.43, 0.58, 0.77
05 Rutin 0.08 24 Toluene + 50% ethyl acetate-2 0.31, 0.45, 0.54,0.63
06 Epicatechin 0.50 25 Toluene + 50% ethyl acetate-3 0.31, 9.37, 0.45, 0.48, 0.54, 0.57, 0.62, 0.68, 0.73
07 Catechin 0.54 26 Toluene + 50% ethyl acetate-4 0.31, 0.38, 0.43, 0.57, 0.71
09 Caffeic acid 0.63 28 Toluene + 75% ethyl acetate-1 0.09, 0.32, 0.50, 0.57
10 Ursolic acid Nil 29 Toluene + 75% ethyl acetate-2 0.81
11 100%Toluene-1 0.81 30 Toluene + 75% ethyl acetate-3 0.1, 0.18, 0.23, 0.28, 0.38, 0.55
12 100% Toluene-2 0.76 31 100% ethyl acetate 0.08, 0.53
13 10%Toluene- 0.67 32 5%methanol + ethyl acetate —
14 10%Toluene-2 0.68, 0.76 33 15%methanol + ethyl acetate —
15 Toluene + 10% ethyl acetate 0.77, 0.61 34 25% methanol + ethyl acetate-1 0.10, 0.30, 0.48, 0.52, 0.61
16 Toluene + 10% ethyl acetate 0.61, 0.69, 0.78 35 25%methanol + ethyl acetate-2 0.06
17 Toluene + 10% ethyl acetate 0.73 36 25%methanol + ethyl acetate-3 0.32, 0.63
18 Toluene + 10% ethyl acetate 0.73 37 25%methanol + ethyl acetate-4 —
19 Toluene + 20% ethyl acetate 0.73, 0.81 38 50%methanol + ethyl acetate- 0.63

The following were used to identify the standards (1–10): Track No. 31 (100 percent ethyl acetate elute) was connected to ellagic acid (RF, value: 0.53); Track No. 13 (10% ethyl acetate in toluene) was connected to kaempferol (RF, value: 0.67); Track No. 20 (25% ethyl acetate in toluene elute) and quercetin (Rf value: 0.62) were identified; Track No. 28 (75% ethyl acetate in toluene elute) was paired with epicatechin (RF, value: 0.57); and rutin corresponded to Track No. 5 (Toluene + 50% ethyl acetate-2, elute), with an Rf value of 0.08.

Rf value was shown by bold values. Ethyl acetate fraction contained active substances and standard biomarkers were matched.