TABLE 5.

Stability of almonertinib, apixaban and rivaroxaban in rat plasma under different conditions (n = 6).

Analytes	Conditions	Concentration (ng/mL)	Mean ± SD (ng/mL)	Precision (RSD%)	Accuracy (RE%)
almonertinib	Autosampler for 12 h	1	0.96 ± 0.05	5.4	−3.88
		80	79.75 ± 4.51	5.7	−0.31
		150	141.00 ± 7.48	5.3	−6.00
	Room temperature for 8 h	1	1.01 ± 0.08	7.7	0.97
		80	80.48 ± 6.42	8.0	0.60
		150	147.50 ± 9.83	6.7	−1.67
	−80°C for 30 days	1	1.02 ± 0.10	10.1	2.33
		80	80.52 ± 3.47	4.3	0.65
		150	146.67 ± 10.63	7.3	−2.22
	Freeze-thaw stability for three times	1	1.01 ± 0.09	8.7	1.47
		80	81.7 ± 2.27	2.8	2.13
		150	146.17 ± 6.68	4.6	−2.56
apixaban	Autosampler for 12 h	2	2.01 ± 0.11	5.5	0.25
		400	423.83 ± 13.30	3.1	5.96
		800	812.17 ± 42.40	5.2	1.52
	Room temperature for 8 h	2	2.08 ± 0.07	3.3	3.83
		400	424.33 ± 9.35	2.2	6.08
		800	804.83 ± 20.23	2.5	0.60
	−80°C for 30 days	2	2.02 ± 0.08	3.8	0.83
		400	400.50 ± 19.99	5.0	0.13
		800	782.83 ± 22.19	2.8	−2.15
	Freeze-thaw stability for three times	2	1.93 ± 0.13	6.8	−3.42
		400	410.67 ± 12.03	2.9	2.67
		800	800.33 ± 24.72	3.1	0.04
rivaroxaban	Autosampler for 12 h	10	10.75 ± 0.48	4.5	7.50
		800	791.33 ± 38.84	4.9	−1.08
		1,500	1,416.67 ± 46.33	3.3	−5.56
	Room temperature for 8 h	10	10.78 ± 0.59	5.4	7.75
		800	833.50 ± 49.92	6.0	4.19
		1,500	1,483.33 ± 72.30	4.9	−1.11
	−80°C for 30 days	10	10.68 ± 0.22	2.1	6.83
		800	765.00 ± 58.14	7.6	−4.38
		1,500	1,435.00 ± 96.07	6.7	−4.33
	Freeze-thaw stability for three times	10	10.11 ± 0.61	6.0	1.13
		800	774.33 ± 23.28	3.0	−3.21
		1,500	1,416.67 ± 48.85	3.5	−5.56