TABLE 2.
Characteristics of OFX-selected mutants of M. hominisa
| Group and strain | MIC (μg/ml)
|
Mutationb in QRDR of:
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| NFX | PFX | OFX | CFX | SFX | GyrA | GyrB | ParC | ParE | |
| Parent M. hominis PG21 | 8 | 2 | 0.5 | 0.2 | 0.01 | ||||
| First-step mutants IO1–IO6 | 16 | 8 | 2 | 1 | 0.02 | None | None | None | Asp426→Asn |
| Second-step mutants IIO1–IIO6 | 32 | 16 | 4 | 2 | 0.05 | None | None | None | Asp426→Asn |
| Third-step mutants | |||||||||
| IIIO316 | 64 | 16 | 32 | 16 | 4 | Ser83→Leu | None | None | Asp426→Asn |
| IIIO58 | 32 | 16 | 8 | 2 | 0.1 | None | None | None | Asp426→Asn |
| IIIO516 | 64 | 16 | 32 | 16 | 2 | Ser83→Leu | None | None | Asp426→Asn |
| IIIO68 | 32 | 16 | 8 | 2 | 0.1 | None | None | None | Asp426→Asn |
| IIIO616 | 32 | 16 | 32 | 8 | 2 | Ser83→Leu | None | None | Asp426→Asn |
| Fourth-step mutants | |||||||||
| IVO3 | 64 | 16 | 32 | 8 | 1 | Ser83→Leu | None | Asp69→Tyr | Asp426→Asn |
| IVO5 | 64 | 32 | 32 | 16 | 4 | Ser83→Trp | None | None | Asp426→Asn |
a
Laboratory strains IO1 to IVO5 were derived from parent wild-type strain PG21 by stepwise selection.
b
M. hominis GyrA, ParC, and ParE residues are numbered in accordance with the E. coli GyrA, ParC, and GyrB coordinates.