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. 2023 Aug 7;10(29):2302298. doi: 10.1002/advs.202302298

Figure 1.

Figure 1

Upregulation of ZBED6 in sepsis‐induced muscle loss for humans and pigs. A) mRNA expression of ZBED6 in muscle biopsies of 25 sepsis patients with muscle loss and 15 orthopedic controls. B) Immunoblot and densitometric analysis of ZBED6 in rectus femoris muscle tissues of sepsis patients and controls (n = 6). Representative images are shown. C) The rectus femoris cross‐sectional area (RF‐CSA) was measured using ultrasound imaging in sepsis patients and orthopedic controls. The area was outlined by a dotted line. Scatterplots showing correlations of ZBED6 transcript with RF‐CSA in sepsis patients and orthopedic controls. D) H&E staining of histological cross sections in rectus femoris muscle tissues of sepsis patients and controls. Representative images are shown. Scale bar = 200 µm. The mean minimum Feret diameter of muscle fibers from sepsis patients and controls were correlated with ZBED6 transcript expression. E) Scatterplots showing correlations of ZBED6 transcript with urea‐to‐creatinine ratio in sepsis patients and controls. F) Scatterplots showing correlations of ZBED6 transcript with the transcripts of FBXO30 and FBXO32 in the rectus femoris muscle tissues of sepsis patients and those of controls. G) Bama pigs were subjected to sham or modified CLP surgery (n = 6). mRNA expression of ZBED6 in skeletal muscles of sham and modified CLP pigs 14 days post‐surgery. (H) Immunoblot and densitometric analysis of ZBED6 in skeletal muscle from sham and modified CLP pigs (n = 3). I) Scatterplots showing correlations of ZBED6 transcript with urea‐to‐creatinine ratio in the sham and modified CLP pigs. J) Scatterplots showing correlations of ZBED6 transcript with FBXO32 and FBXO30 in skeletal muscle of sham and modified CLP pigs. Data are expressed as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001.