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. 2023 Aug 7;10(29):2302298. doi: 10.1002/advs.202302298

Figure 4.

Figure 4

Loss of ZBED6 inhibits protein breakdown during sepsis‐induced muscle atrophy in pigs. A,B) Plasma urea concentration (A) as well as urea‐to‐creatinine ratio (B) in sham or modified CLP pigs 14 days post‐surgery. ZBED6‐deficient pigs (CLP, n = 6; sham, n = 6), WT (CLP, n = 6; sham, n = 6). C–E) Relative mRNA expression of markers for atrophy in TB (C), LD (D), and EDL (E) muscles. F) Western blot analysis of muscle atrophy genes in EDL muscles from sham and CLP pigs. (Right panel) Quantification of the p‐Foxo3A/Foxo3A, MURF1, and lysine 48 poly‐ubiquitinated protein levels against internal control α‐Tubulin. G) Representative images (Left) and quantification (Right) for SUnSET puromycin incorporation assay in muscles from sham and CLP pigs 14 days after surgery. ZBED6‐deficient pigs (CLP, n = 3; sham, n = 3), WT (CLP, n = 3; sham, n = 3). Data are expressed as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001.