TABLE 1.

Properties of resistant clinical isolates of S. pneumoniae

Isolate no.	Serotype/clonea	Antibiogramb	PCR result			Size(s) (kb) of AvaI fragment(s) in Tn917c
			ermAM	tet(M)	aphA-3
E1	23F/Spanish	PN, CM, TC, EM, CC	+	+	−	2.3, 1.60, 1.27
E2	23F/Spanish	PN, CM, TC, EM, CC	+	+	−	2.3, 1.75, 1.27
E16	6B/Spain-Iceland	PN, CM, TC, EM, CC	+	+	−	2.3, 1.75, 1.27
1003	Nontypeable	PN, TC, EM, CC	+	+	−	2.3, 1.75, 1.27
E41	Nontypeable	PN, TC, EM, CC	+	+	−	2.3, 1.75, 1.27
BM4200	Nontypeable	PN, CM, TC, EM, CC	+	+	+	22.5
E6	23F/Spanish	PN, CM, TC, EM, CC	+	+	−	16
E12	6B/Spain-Iceland	PN, CM, TC, EM, CC	+	+	−	22
E42	19A	PN, EM, CC	+	+	−	22
E43	6B/Spain-Iceland	PN, TC, EM, CC	+	+	−	19
3543	6B/Spain-Iceland	PN, CM, TC, EM, CC	+	+	−	17.5
2649	6B/Spain-Iceland	PN, CM, TC, EM, CC	+	+	−	22

^aClonal type relatedness was determined by PFGE. 

^bThe antibiogram indicates resistance to the indicated drugs. The resistance breakpoints were as follows: penicillin (PN), MIC ≥ 2 μg/ml; chloramphenicol (CM), MIC ≥ 8 μg/ml; tetracycline (TC), MIC ≥ 8 μg/ml; erythromycin (EM), MIC ≥ 1 μg/ml; clindamycin (CC), MIC ≥ 1 μg/ml. 

^cMolecular size(s) of AvaI-digested genomic DNA fragments hybridized with digoxigenin-labeled Tn917.