REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Galectin-3	Abcam	Cat#ab76245; Clone: EP2775Y; RRID: AB_2265782
Ki-67	Abcam	Cat#ab16667; Clone: SP6; RRID: AB_302459
Cytokeratin 19 (CK19)	Affinity Biosciences	Cat#AF5106; RRID: AB_2837592
Cy3-labeled goat anti-rabbit IgG (H + L)	Beyotime	Cat#A0516; RRID: AB_2893015
Biological samples
Human papillary thyroid cancer tissues	This study	N/A
Chemicals, peptides, and recombinant proteins
B27	Gibco	Cat#17504-044
N-acetyl-L-cysteine	Sigma-Aldrich	Cat#A9165
Nicotinamide	Sigma-Aldrich	Cat#N0636
R-spondin-1	Peprotech	Cat#120-38
Noggin	Peprotech	Cat#120-10C
SB202190	Sigma-Aldrich	Cat#S7067
A83-01	Sigma-Aldrich	Cat#SML0788
EGF	Peprotech	Cat#AF-100-15
FGF-7	Peprotech	Cat#100-19
FGF-10	Peprotech	Cat#100-26
Y-27632 dihydrochloride	Abmole	Cat#M1817
Sodium alginate	Sigma-Aldrich	Cat#71238
Gelatin	Sigma-Aldrich	Cat#73865
Calcium chloride	Macklin	Cat#C805225
Cupric chloride	Macklin	Cat#C805298
Critical commercial assays
CellTiter-Glo 3D Reagent	Promega	Cat#G9683
Cell Counting Kit-8	MedChemExpress	Cat#HY-K0301
Reactive Oxygen Species Assay Kit	Beyotime	Cat# S0033S
Calcein-AM	Biolegend	Cat#425201
Propidium iodide	Biolegend	Cat#421301
Experimental models: Cell lines
BCPAP	Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, China	Cat#SCSP-543
8305C	Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, China	Cat#SCSP-540
CAL-62	Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, China	Cat#SCSP-546
K1	iCell Bioscience Inc., Shanghai, China	Cat#iCell-h394
FTC-133	Cobioer Biosciences Co., Ltd., Nanjing, China	Cat#CBP61109
TPC-1	Cobioer Biosciences Co., Ltd., Nanjing, China	Cat#CBP60257
Experimental models: Organisms/strains
Human papillary thyroid cancer organoids	This study	N/A
Software and algorithms
Prism GraphPad 7.0	GraphPad Software, Inc., CA, USA	N/A
SPSS 19.0	SPSS, Inc., IL, USA	N/A
OriginPro 8.0	OriginLab Corporation, Northampton, MA, USA	N/A
CorelDraw X7	Corel Corporation, Ottawa, Canada	N/A