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. 2023 Aug 10;16(10):1957–1971. doi: 10.1111/cts.13604

FIGURE 5.

FIGURE 5

TXNDC12 regulates SLC7A11 by directly interacting with SLC7A11. (a) Silver staining of affinity protein purification complexes of the Flag‐TXNDC12 U251 stable cell line and parent U251 cell line. (b) The TXNDC12 expression vector was transfected into U251 cells in the presence or absence of Flag‐SLC7A11, and TXNDC12 was detected in the immunoprecipitation complex of Flag‐SLC7A11. (c) SLC7A11 expression vector was transfected into U251 cells in the presence or absence of Flag‐TXNDC12, and SLC7A11 was detected in the immunoprecipitation complex of Flag‐TXNDC12. (d) After co‐immunoprecipitation of endogenous TXNDC12 from U251 cells, endogenous SLC7A11 was analyzed by Western blotting. (e) After co‐immunoprecipitation of endogenous SLC7A11 from U251 cells, endogenous TXNDC12 was analyzed by Western blotting. (f) Interaction between TXNDC12 and SLC7A11, as detected by a GST‐pull down experiment. (g) TXNDC12 and SLC7A11 expression in U251 and LN229 cells, as detected by an immunofluorescence assay. The merged images show the overlays of TXNDC12 (red) and SLC7A11 (green). The nuclei were stained with DAPI (blue). Scale bar: 20 μm.