TABLE 2.
Time course of effect of ganciclovir on the percentage of antigen-positive V379354-infected cells
| Time (h) postin- fection | Ganciclovir concn (μM) | % IE Ag + cellsa | IC50 (μM) for IE Ag + cellsb | % Late Ag + cellsc | IC50 (μM) for late Ag + cells |
|---|---|---|---|---|---|
| 24 | 0 | 16.9 | 2.0 | ||
| 6 | 14.3 | 1.9 | |||
| 12 | 15.7 | 1.8 | |||
| 48 | 0 | 34.1 | 2.1 | ||
| 6 | 23.2 | 1.4 | |||
| 12 | 20.2 | 1.4 | |||
| 72 | 0 | 64.1 | 3.3 | ||
| 6 | 34.5 | 6.61 | 1.8 | 7.84 | |
| 12 | 26.5 | 1.6 | |||
| 96 | 0 | 78.3 | 4.3 | ||
| 6 | 27.8 | 4.21 | 1.7 | 4.64 | |
| 12 | 23.4 | 1.4 | |||
| 120 | 0 | 83.1 | 19.4 | ||
| 6 | 39.1 | 5.55 | 4.0 | 3.31 | |
| 12 | 31.8 | 3.2 | |||
| 144 | 0 | 77.2 | 26.2 | ||
| 6 | 39.2 | 6.12 | 6.9 | 4.21 | |
| 12 | 27.8 | 3.2 |
a
IE Ag + cells, immediate-early-antigen-positive cells. The percentage of antigen-positive cells was derived from one flow cytometric analysis.
b
IE Ag, immediate-early antigen.
c
Late Ag + cells, late-antigen-positive cells. The percentage of antigen-positive cells was derived from one flow cytometric analysis.