TABLE 3.
Comparison of results of flow cytometry and plaque reduction assays
| Clinical isolate | IC50 (μM)
|
Ganciclovir phenotypea | |
|---|---|---|---|
| Flow cytometry assayb | Plaque reduction assayc | ||
| V917401 | >96 | >96 | R |
| MR11979 | >96 | >96 | R |
| OBR | >96 | 20 | R |
| GU | >96 | 13.2 | R |
| MAL | 8.48 | 7.93 | PR |
| K8313 | 9.79 | 9.21 | PR |
| V379354 | 3.27 | 5.52 | S |
| SAG | 6.66 | 5.65 | S |
| MG | 4.51 | 5.00 | S |
| VIL | 1.14 | 1.62 | S |
| TUC | 1.28 | 1.86 | S |
| GRO | 5.72 | 3.05 | S |
| LAN | 4.20 | 2.26 | S |
| COP | 6.65 | 3.62 | S |
| MAC | 6.30 | 4.02 | S |
| BRO | 6.53 | 4.20 | S |
| PHI | 3.91 | 1.32 | S |
| LAP | 4.82 | 2.33 | S |
| HOW | 1.39 | 2.18 | S |
| PER | 1.75 | 2.27 | S |
| MCI | 4.54 | 2.30 | S |
| LA | 2.48 | 1.17 | S |
| JD | 5.50 | 3.00 | S |
| FK-1 | 6.28 | 1.75 | S |
| FK-3 | 5.13 | 1.78 | S |
a
The phenotype was based on the results of the plaque reduction assay. R, resistant; PR, partially resistant; S, sensitive.
b
Data are from a single analysis.
c
Data are from a single plaque reduction assay performed in quadruplicate. The well-to-well variation in the numbers of PFU at the same drug concentration was less than 10%.