Figure 4.

Comparison of rhythmic genes in healthy liver and tumors. A, Venn diagram illustrating overlap in genes identified as rhythmic in liver and tumors. B–D, Heat maps showing the rhythmicity, in both liver and tumor, of genes that are categorized in (A) as rhythmic only in liver (B), rhythmic in both liver and tumor (C), and rhythmic only in tumor (D). Below each heat map is an associated radial diagram where appropriate. In the heat maps, the black/white bar beneath each indicates dark/light periods, and data for the 12 time points are above the bars ordered successively from ZT0 to ZT24 left to right. Data for the different genes are ordered from top to bottom based on time of maximal expression (yellow is the highest). Relative expression of each gene was determined by plotting, for each time point, the Exp/Med, or the experimental value for each time point divided by the median value for all time points. The radial diagrams illustrate with bars the number of genes with peak expression at each ZT. Background shading in grey identifies the dark hours between ZT12 and ZT0.