Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Oct 17;9(4):e003255. doi: 10.1136/rmdopen-2023-003255

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Single-cell RNA sequencing identifies distinct chondrocyte subsets in the cartilage from weight-bearing (iMT) and non-weight-bearing (oLT) sites in patients with osteoarthritis (OA). (A) Visualisation of chondrocytes subsets by Uniform Manifold Approximation and Projection (UMAP) plot (left panel). Feature plots show the expression distribution of subset-specific markers for each of the seven chondrocyte subsets (clusters 1–3, 8–11) on the UMAP plot (middle panel). Violin plots showing the expression levels of these markers, the grey dashed line highlights a comparatively higher average expression level relative to other subsets (right panel). (B) Visualisation of the spatial distribution of annotated chondrocyte subsets on the UMAP plot. (C) The percentage distribution of stable chondrocytes, hypertrophic chondrocytes, endothelial cells, osteoarthritis hypertrophic chondrocytes (OAHCs), fibrochondrocytes, and chondrogenic progenitor cells in cartilage tissues under different weight-bearing conditions. (D) Milo analysis reveals a pronounced enriched associated with weight-bearing conditions in the abundance of cell neighbourhoods in the OAHCs compared with other chondrocytes. (E) Heatmap of biological process significantly associated with the transcriptome of each of the six annotated chondrocytes subsets as identified using DAVID functional annotation tool, and coloured by scaled gene number. (F) Heatmap of significant pathways for each of the six chondrocytes subsets as identified using Reactome Pathway Database, and coloured by scaled gene number. (G) DotPlot depicts the expression pattern of differentially expressed proteins identified through Tandem Mass Tag proteomics analysis in each of the annotated chondrocyte subsets. The size of the circles represents the proportion of protein expression in the corresponding subset, while the colour indicates the average expression level.