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. 2023 Sep 27;26(10):108079. doi: 10.1016/j.isci.2023.108079

Figure 2.

Figure 2

Dexamethasone downregulates GM-CSF-induced IRF4 expression in human monocytes and mouse macrophages

(A and B) Human monocytes were treated with either GM-CSF (20 ng/mL) and Dex (100 nM) alone or together for 16 h. A) IRF4, IRF5, and IRF8 mRNA expression (n = 8) and B) protein expression in whole cell lysates (western blotting) with anti-IRF4, anti-IRF8, and anti-β-actin antibodies (n = 4).

(C and D) Human monocytes were pre-treated with mifepristone (1μM) for 30 min before treated with GM-CSF alone or together with Dex for 16 h. C) IRF4 mRNA expression (n = 4) and D) IRF4 and β-actin protein expression in whole cell lysates (n = 4).

(E and F) BMM were treated with GM-CSF (20 ng/mL) and Dex (100 nM) alone or together for 16 h. E) Irf4 mRNA expression (n = 5) and F) IRF4 and β-actin protein expression in whole cell lysates (n = 4). The data are graphed as scatterplots with bars indicating mean ± SEM. p values were obtained using one-way ANOVA with Tukey post-test, where ∗p < 0.05, ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001. Original Western blots are presented in Figure S1.