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. 2023 Sep 27;26(10):108079. doi: 10.1016/j.isci.2023.108079

Figure 4.

Figure 4

Dexamethasone ameliorates arthritic pain-like behavior and disease, correlating with decreased CCL17 expression in joints

(A–C) mBSA/GM-CSF arthritis (mBSA i.a.; day 0) and s.c. saline or GM-CSF (500 ng, days 0–2) or i.p. Dex (0.25 mg/kg, at day −2, 0 and 2) was induced in WT C57BL/6 mice. A) Arthritic pain-like behavior (incapacitance meter; n = 5/group), B) disease (day 7, histology – H&E stain; n = 5/group) and (C) joint Ccl17 mRNA expression (day 7, qPCR; n = 4/group) were measured.

(D–F) Zymosan-induced arthritis (300 μg zymosan i.a.; day 0) or i.p. Dex (0.2 mg/kg, at day −1, 1 and 4) was induced in WT C57BL/6 mice. D) Arthritic pain-like behavior (incapacitance meter; n = 7/group), E) disease (day 7, histology – H&E stain and Safranin O/Fast Green stain; n = 5/group) and (F) joint Ccl17 mRNA expression (day 7, qPCR; n = 7/group) were measured. The data are graphed with bars indicating mean ± SEM. p values were obtained using two-way ANOVA with either Dunnett post-test (A) or Sidak post-test (D), one-way ANOVA with Tukey post-test (B, C), or unpaired t test (E, F), where ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001. Scale bar represents 25 μM.